Molecular Biology: Gene Expression Techniques and Cancer


Created Anum Ahmad, PhD | Last updated 10/2021
Duration: 1h 28m | 9 sections | 22 lectures | Video: 1280×720, 44 KHz | 745 MB
Genre: eLearning | Language: English + Sub

Learn about gene expression analysis techniques and their applications in diagnosis and treatment of cancer.
What you’ll learn

Gene and its components

What is gene expression

Introduction to gene expression techniques

Techniques for mRNA expression analysis

Introduction to hybridization based methods

Northern blot

RNA-FISH

DNA microarray

Introduction to PCR based methods

RT-PCR

Real time qRT-PCR

Introduction to sequencing based techniques

Sanger method

High-throughput sequencing technology

Serial Analysis of Gene Expression (SAGE)

RNA sequencing

Applications of gene expression analysis

What is cancer

Oncogenes and tumor suppressor genes

Challenges in understanding cancer genetics

Techniques used to study cancer

Targeted therapy for cancer

Requirements

Basic knowledge of biology and molecular biology

Interested in learning latest technologies

Description

The field of molecular biology has a profound impact in life science investigation. The development of more and more sophisticated experimental techniques in molecular biology with a broad, interdisciplinary applicability have stimulated research and progress in almost all the disciplines of life science. The rationality and approach of scientific experimentations have changed and allowing revolutionizing discoveries not only in molecular biology but also in biochemistry, biophysics, biotechnology, cell biology, and genetics. Reserachers can choose suitable techniques based on their usefulness, objectives and the financial and temporal resources available to the them. Many molecular biology techniques have extensively been used in the study of gene expression.

Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product (mRNA) that enables it to produce end products (protein), and ultimately affect a phenotype, as the final effect.

The technique

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